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Centrifuge 5418 / 5418 R
18-place capacity centrifuges for molecular biology that are specifically designed for low to medium capacity in 1.5/2.0 mL tubes and Microtainer®. Their speed of up to 16,873 × g allows for standard molecular biology applications.
The Eppendorf Centrifuge 5418 is a compact 18-place centrifuge designed for labs with fewer samples but high demand in quality and performance. With the product technical this microcentrifuge covers all the application in todays' labs.
Common Features of Centrifuge 5418 and refrigerated 5418 R:
Equipped with an aerosol-tight 18 x 1.5/2.0 mL tubes rotor by standard
Unique QuickLockT rotor lid
Remarkably quiet operation – even without the QuickLock � rotor lid – to improve your work environment
Speed up to 16,837 x g (14,000 rpm)
Very low access height for easy loading and unloading
Separate ShortSpin button for fast and convenient quick spins
Separate rpm/rcf converter button
Rotary knob operation for fast selection of parameters
Additional product features of refrigerated Centrifuge 5418 R
Patented compressor technology minimizes vibrations – protects your precious sample!
Temperature range: 0 °C to +40 °C
Low access height of 26 cm, as refrigeration unit is at the rear of the unit
Maintains constant 4 °C at max. speed
Fast Temp for rapid pre-cooling of the centrifuge
Continuous cooling maintains temperature when centrifugation process is not active
Yes, the device has an emergency lid release. Before opening the centrifuge, pull the power plug and wait until the rotor stops spinning. The centrifuge 5418 has an emergency lid release via a cord that is located on the underside of the device in the base plate. Remove the white plastic cap and pull the cord downwards. The centrifuge 5418 R has an emergency lid release behind a plastic cover on the right side of the device. Rotate the plastic cover counterclockwise and remove it. Enter the centrifuge rotor key into the hexagonal opening at rear until some resistance can be felt. Slightly press and turn the rotor key counterclockwise.
The Centrifuge 5418/5418 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method. A detailed description can be found in the operating manual, which can be downloaded from our website.
In the centrifuge 5418, for a run time up to 10 min the increments are 0.5 min steps, after that it is set in increments of 1 min up to 9:59 h. The function for continuous run mode can be found above 9:59 h and below 0.5 min The symbol "oo" is displayed to indicate the continuous run. In the centrifuge 5418 R, for a run time up to 10 min the increments are 0.5 min steps, after that it is set in increments of 1 min up to 99 min. The function for continuous run mode can be found above 99 min and below 0.5 min. The symbol "oo" is displayed to indicate the continuous run.
"EP" is displayed when the automatic standby function is active. It is not an error code. If the centrifuge remains unused for more than 15 min., the standby function is activated, indicated by "EP" on the display. By using any key or knob or closing the centrifuge lid, the centrifuge is re-activated and ready to use. The letters "EP" were chosen because a lot of Eppendorf products already have this prefix in their name.
The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.
The fixed-angle rotor is made of massive, anodized aluminum. This material is durable and has got a good chemical resistance. The rotor lid is made of the same material.
The sealing of the aerosol-tight rotor FA-45-18-11 can very easily be exchanged. A detailed description with illustrations can be found in the operating manual, which can be downloaded from our website.
1.5 ml and 2.0 ml Eppendorf<sup>®</sup> tubes can be spun in the Centrifuge 5418/5418 R. With the help of adapters, the centrifugation of 0.2 ml PCR tubes, 0.6 ml Microtainer, 0.5 ml and 0.4 ml micro tubes is also possible. Even so-called spin columns (e.g. Quiagen, Macherey-Nagel, Vivascience) can be centrifuged. For centrifugation of spin columns, the rotor lid must be used.
The rotor of the Centrifuge 5418/5418 R allows a broad variety of applications. Up to 18 micro<br />test tubes can be centrifuged at 16,873 x g. It can be used with tubes from 0.2 up to 2.0 ml. The<br />potential applications include:<br />- Isolation of DNA, RNA and proteins<br />- Spin columns<br />- Aerosol-tight centrifugation of infectious samples<br />- Pelletizing of cells (bacteria, eukaryotic cells) up to 2.0 ml<br />- Spinning down of PCR mixtures in tubes
Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA”, where the letter “A” indicates the aerosol tightness; secondly, we have color-coded the aerosol-tight rotors. All aerosol-tight rotors now have a red rotor lid screw, and a red ring is printed on to the rotor shaft.
At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.
Following the end of a centrifuge run, only the lid of the Centrifuge 5418 is automatically opened in<br />order to provide quicker access to the sample and to avoid potential heat accumulation. The Centrifuge 5418 R is not equipped with the automatic opening of the centrifuge lid. In order to maintain a consistent temperature in the rotor chamber and in the sample, the centrifuge lid for this device must always be manually opened by pressing the "open" key.
The Centrifuge 5418 can be operated at an ambient temperature in the range from 2° C to 40° C. Operation in the cooling room at a temperature of 4° C is therefore possible. The permitted range of the ambient temperature is between 15 °C and 35 °C for the Centrifuge 5418 R, so that this centrifuge could not be operated in a cooling room at 4 °C.
The "Fast Temp" function is a temperature control run of the Centrifuge 5418 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and g force (rcf) or speed (rpm) as well as “FT” as symbol for a “Fast Temp” temperature run. You can find a more detailed description in the operating manual of the Centrifuge 5418/5418 R.
The continuous cooling starts with each rotor standstill of the Centrifuge 5418 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:<br />- The centrifuge is switched on.<br />- The centrifuge lid is closed.<br />- The nominal temperature is lower than the ambient temperature.<br />Continuous cooling is ended by opening the centrifuge lid.
The lowest temperature of the Centrifuge 5418 R is 0 °C. The device is thereby optimized with the rotor in such a way that at least 4 °C is maintained at the maximum speed.
No, in order to ensure the full functionality of the lid latch, it must not be cleaned or greased. If you feel that this is nevertheless necessary, please contact our Technical Service. The contact address can be found in the operating manual of your centrifuge.
Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.
