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EPPENDORF Research® plus
The ultra light Eppendorf Research plus pipette meets the highest needs in precision and accuracy – combined with ultimate ergonomics and increased flexibility.
Eppendorf sets a new standard in pipetting. The ultra light adjustable Eppendorf Research plus pipette meets the highest needs in precision and accuracy – combined with ultimate ergonomics and increased flexibility.
It is a good feeling to know you are doing the right thing – to know you are working with the most advanced pipette in the world. A spring loaded tip cone, a secondary calibration option, an improved volume display – and all that in an ultra light, fully autoclavable pipette: That is the new Eppendorf Research plus pipette.
In Australia its an even better feeling to know that you are dealing with the strongest link in the supply chain – POCD Scientific’s sales consultants know this instrument inside out and can offer you the best deal in terms of price, supply, expertise and knowledge.
Ergonomics: The more strain you experience, the less energy you have. And because energy is a very precious and exhaustible resource, we try to use it as efficiently as possible. Thus the Eppendorf Research plus has been developed within the strict criteria of the Eppendorf PhysioCare Concept®.PhysioCare Concept® stands for ultimate durability, intuitive operation and above all for perfect balance and minimal user exertion.
Flexibility: Your next experiment will need new pipetting schemes. Your new laboratory might have different sources of contamination. Your new pipette should offer all the flexibility you need. Calibrate your Research plus to your needs, autoclave the entire pipette or only the lower part. Choose among single channel, multi channel and fix-volume pipettes in different sizes.
Robustness: Durability was tested thoroughly and has met the highest quality requirements. Resistance to chemical, thermal and mechanical stress is now unchallenged!
Features
Volume adjustment: Only a few turns to reach your desired volume
Control button: Very low operating force, colour indicates pipette volume, positioned for perfect ergonomics
Ejector: Very low operating force, positioned for perfect ergonomics
Volume display: 4 digits, magnifying shape
Calibration display & adjustment opening: Calibrate your pipette to a specific liquid and volume
Eppendorf PerfectPiston™: Ultra light piston made of Fortron® (not in 2.5 µL, 10 µL, 5 mL and 10 mL pipettes)
Quick connection clip: Remove lower part easily (not in 5 mL and 10 mL pipettes)
Spring loaded tip cone: Improved ergonomics, tight fit to the tip (not in 5 mL and 10 mL pipettes)
Technical Information
Volume range
Volume
Systematic error
Random error
Operating button anthracite for pipette tips 10 µL
0.1 – 2.5 µL
0.1 µL
±48.0 %
±0.048 µL
±12.0 %
±0.012 µL
0.25 µL
±12.0 %
±0.03 µL
±6.0 %
±0.015 µL
1.25 µL
±2.5 %
±0.031 µL
±1.5 %
±0.019 µL
2.5 µL
±1.4 %
±0.035 µL
±0.7 %
±0.018 µL
Operating button grey for pipette tips 20 µL
0.5 - 10 µL
0.5 µL
±8.0 %
±0.04 µL
±5.0 %
±0.025 µL
1 µL
±2.5 %
±0.025 µL
±1.8 %
±0.018 µL
5 µL
±1.5 %
±0.075 µL
±0.8 %
±0.04 µL
10 µL
±1.0 %
±0.1 µL
±0.4 %
±0.04 µL
Operating button light grey for pipette tips 20 µL L
Reverse pipetting means uptake of liquid including the blow-out volume and dispensing of liquid without the blow-out volume. Reverse pipetting is recommended to reduce the error with viscous or foaming liquids or when dispensing very small sample volumes. The excess solution aspirated remains in the tip and is discarded.
When pipetting viscous liquids, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).
When pipetting chloroform, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).
Diethyl ether has such a high vapour pressure that it would leak out of the tip immediately when an air cushion pipette is used. For this reason, it can only be pipetted using a positive displacement pipette, such as Biomaster, Multipette/Repeater, and Varipette.
A 99 % solution of n-amyl alcohol is a highly viscous solution. We recommend working with an instrument that operates according to the positive displacement principle like Multipette/Repeater, Varipette, or Biomaster.
It is best to use a positive displacement device like the Multipette/Repeater. Positive displacement devices are most suitable for precise and drip-free dispensing of liquids with high vapour pressure. As the liquid is enclosed in a tip with integrated piston, the solvent vapour does not come into direct contact with the instrument.
Both dichloromethane and n-hexanol have a very high vapour pressure. For that reason, positive displacement instruments like the Biomaster and the Multipette/Repeater should be used.
No, we cannot guarantee this. Of course we work carefully and cleanly in production, but no special measures are taken to prevent the infiltration of nucleic acids. To protect your pipette from contamination, we recommend using positive displacement pipettes, for example, our Biomaster or filtertips (ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax).
Following contamination of pipettes with radioactive liquids, we recommend placing the contaminated parts in a complex-forming liquid or special cleaning solution. Then rinse very thoroughly with distilled water and dry. Grease piston lightly afterwards.
Exactly in the same way as liquids at room temperature, however, please note that solutions may behave differently. In addition, the pipetting accuracy / precision may be outside the permissible error limits. In order to achieve maximum accuracy / precision, all components should have the same temperature: pipette, tips, liquid, and the surrounding air.
Temperature effects are minimised in our pipettes by their design. We use a special polypropylene which satisfies the special requirements on a pipette handle.
This belongs to the permissible errors of your specific application. In general an adjustment of air cushion pipettes, in other words altering the stroke of the pipette, is required for solutions of a density größer 1.2 mg/µL. <br />Please be aware that variable manual pipettes turn into "fixed-volume pipettes" when being adjusted: They can only be adjusted to a specific liquid to one volume and not across the entire dispensing range.<br />Eppendorf manual pipettes have a user adjustment for temporary adjustment purposes. This user adjustment can easily be set back to manufacturer settings without the need for recalibration. For the correct procedure and examples for settings of user adjustments for different liquids please see the operating instructions and CD enclosed to your pipette.
The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.
ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters:<br />ISO 8655-1: Terminology, general requirements, and user recommendations<br />ISO 8655-2: Piston pipettes<br />ISO 8655-3: Piston burettes<br />ISO 8655-4: Dilutors<br />ISO 8655-5: Dispensers<br />ISO 8655-6: Gravimetric method for the determination of measurement error<br />ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)
The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.
The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig.<br />The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.
According to the EN ISO 8655 standard, a variable pipette should be calibrated to 3 volumes during conformity testing:<br />1. Nominal volume,<br />2. 50 % of nominal volume,<br />3. Smallest value that can be set or 10 % of nominal volume.
The weight fluctuations you measured would appear to be a temperature problem. Please ensure that the pipette, the distilled water used for testing, the tips etc… are all at the same temperature. It is recommended that the pipettes be placed in the weighing room at least 2 hours before the test is performed. While measuring, ensure that transfer of heat from your hands is kept to a minimum (otherwise weight fluctuations may occur!) Tip: do not constantly grasp the pipette in the palm of your hand while waiting for each measurement result! We recommend our standard operating procedure (SOP) for pipettes as a source of basic information on pipette calibration.
According to ISO 8655, all channels of multi-channel pipettes are to be tested. All channels have to be filled but only the channel to be tested is measured. Because of the high number of measurements (8-channel pipette: 240 measurements, 12-channel pipette: 360 measurements), it makes sense to send the multi-channel pipette to a professional calibration lab. These labs have special constructions for balances to ensure that all channels are filled but only one channel is measured.
To attach the Multipette holder adapter you first need to remove one pipette holder from the pipette carousel. To do this, undo the screw at the top end of the pipette holder. Once the pipette holder has been removed from the carousel you can put the Multipette holder in the free space in the carousel and screw it all firmly together again.
In most cases, concentrated acids are not a problem. Please check the chemical resistance information on our homepage before use. Please note that aggressive chemicals used over a longer period of time may shorten the lifetime of the pipette.
From a technical point of view, a gravimetric test is not required. However, for certified laboratories a gravimetric test might be required for documentation purposes.
Yes. Please notice that the usage of aggressive chemicals like hydrochloric acid over a longer period of time will reduce the life span of the pipette.
Yes, in general, however, discoloration may take place and the piston seal may corrode. Please note that the use of aggressive chemicals like nitric acid over a longer period of time will reduce the lifetime of the pipette.
The user manual of the Research plus mentions specific settings for some volumes of the most common liquid classes. These values are for orientation only as the systematic and random error are affected by many factors like, e.g., the mode of operation, the tip used, temperature. It is essential to check this data according to your own method.
We recommend to dismantle the pipette into its individual parts (see operating instructions) and to clean these. Afterwards calibrate your pipette following the instructions given in our SOP. Values which deviate significantly from the limit value should now no longer occur. However, if there is no contamination, please contact your local eppendorf partner for service of your pipette.
vapour will penetrate the inside of the pipette during autoclaving but this will not cause damage. We tested our pipettes with 175 autoclaving cycles (121 °C, 1 bar, 20 min). The number of tests chosen corresponds to an average of 5 years of use.
For technical reasons, the results are generally more precise when a volume setting is carried out from a higher down to a lower value. This is valid for all manual pipettes.
Wipe the outer surface with disinfectant, DNA/RNA decontamination agent, or 70 % isopropanol. After the exposure time of the disinfectant, rinse thoroughly with demineralized water. This step is especially important if subsequent decontamination, e.g., with UV-light or autoclaving, should be performed. The Research plus may be temporarily subjected to UV light under a sterile bench. We recommend the use of a Hg-lamp, 254 nm, 30 W. The distance between lamp and pipette should be approx. 60 cm. The Research plus is fully autoclavable.
The Research plus pipettes are easy to clean. Only the lower part should be cleaned on a regular basis in accordance with the frequency of use. The lower part of the single-channel and multi-channel Research plus pipettes can be removed easily as described in the operating manual of the Research plus or in our Application Note 198.
Pipette parts which might come in contact with liquids are made of the following materials: PP (polypropylene), PEI (polyetherimide), PVDF (polyvinyliden fluoride), PC (polycarbonate), PPS (polyphenylene sulfide), and stainless steel.
For dispensing tasks in which a pipette tip is used over a long time or is slightly bent, it can be helpful to deactivate the spring loading of the tip cone by installing the locking ring. This can also be helpful for pipette tips which are not designed for Eppendorf pipette cones.
The user decides upon the frequency of calibration, the number of test volumes and the number of measurements for each test volume within the framework of monitoring his test materials. This can vary depending on the frequency of use of the pipette, the number of users of the device, the aggressiveness of the liquids to be dispensed and the error limits deemed acceptable by the user. However, an inspection should be carried out at least once a year. See also EN ISO 8655-6.
