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GALILEO Vertical Gel Electrophoresis Units
These vertical gel electrophoresis units from Galileo are the highest quality electrophoresis devices on the market.
Overview
Choose from four sizes of Galileo Refection™ twin gel vertical electrophoresis devices.
These Galileo Reflection™ vertical units all feature a cast in place sytem for quick, tape-free gel casting. The gel sandwich of glass and spacers is attached to the upper buffer chamber in the normal gel run position.
The upper buffer chamber is then placed on a gasketed casting base to seal the lower edges of the gel.
Gels are then poured as normal, and after the gels have polymerized, the completed gels and upper buffer chamber are transferred to the lower buffer chamber for running.
Find out which unit is right for you – check out the Models tab for further details.
Features
All Models
All models have the features listed below
- Heavy duty upper buffer chamber with cooling port connections.
- Lower buffer chamber.
- Interlock safety lid with attached leads.
- Casting base.
- Four-pack each of plain & notched glass plates.
- One acrylic blocker plate (used when running only one gel)
Model Specific Features
| Model |
Features |
| 1010 |
- 2 × 10-well, 0.8 mm thick combs
- 4 × 0.8mm thick side spacers
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| 2010 |
- 2 × 15-well, 0.8 mm thick combs
- 2 × 20-well, 0.8 mm thick combs
- 8 × 0.8mm thick side spacers
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| 1614 |
- 2 × 10-well, 1.5 mm thick combs
- 2 × 15-well, 1.5 mm thick combs
- 8 × 1.5 mm thick side spacers
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| 2020 |
- 2 × 15-well, 1.5 mm thick combs
- 2 × 20-well, 1.5 mm thick combs
- 8 × 1.5 mm thick side spacers
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Accessories
- Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
- Casting base. For quick, efficient and leak-proof casting.
Note that Galileo mini-gel accessories, including trays and combs, also fit many other gel units from other suppliers.
Automatic Buffer Recirculation
Certain Galileo horizontal electrophoresis units are now available with automatic buffer recirculation.
Galileo automatic buffer recirculation reduces detrimental ion and pH gradients in your buffer, encourages even migration of nucleic acids, and is ideal for long running gels.
Buffer recirculation can also prevent glyoxalated RNA from dissociating in the presence of ion gradients.
The system is simple and self contained.
A trapping system captures hydrogen bubbles created by electrolysis of the buffer at the cathode (-) end of the unit.
As these bubbels move up the canted recirculation tube, they carry buffer along with them and provide a gentle and reliable recirculation system.
This system is self regulating and no additional tubing or pumps are required.
Choose from three fast and efficient, tape-less casting systems from Galileo.
Gel Trays BulletRapidCast™ – cast in place system
Galileo’s RapidCast system is ideal for most small gel units with trays that are approximately equal in width and length. The dimensions of all Galileo cast-in-place gel trays are adjusted so that when the tray is placed cross- ways in the gel tank, the gasketed ends of the gel tray fit precisely against the walls of the buffer chamber. This forms a leak-proof seal for quick gel pouring. This system can also be used with RapidCast casters, for casting outside the buffer tank. A long-lived silicone rubber gasket ensures a reliable seal thru many gel castings and side grooves on all RapidCast trays provide for easy handling. All Galileo trays feature multiple comb slots, built-in fluorescent rulers and ultra-strong assembly methods for long, reliable service.
Gel Trays BulletExpressCast™ – end gated tray system
High thru-put / high resolution systems require gel trays too long to fit cross ways in the gel tank. For easy tape-less casting with these units, Galileo has developed the ExpressCast™ system. ExpressCast trays incorporate removable gasketed end gates. These end gates fit into slots at both ends of the tray, to form a quick leak-proof seal for gel casting. Because the length of ExpressCast trays is not constrained by the width of the gel unit, these trays can accommodate longer run lengths or more sample capacity. Note that for easy upgrading of your old units, Galileo ExpressCast trays are also compatible with gel units from many other manufacturers. All Galileo trays feature multiple comb slots, built-in fluorescent rulers and ultra-strong assembly methods for long, reliable service.
Gel Trays BulletMulti-Cast casters for traditional flush-cut trays
For those wishing to use traditional trays, Galileo also supplies, standard flush-cut trays for many units. The two sizes of adjustable multi-gel casting platforms from Galileo, enable quick leak-proof casting of multiple gels using flush-cut trays. These casters accomodate a wide range of tray sizes and styles, and quickly seal both ends with a simple turn of the accuation cam.
Note that for easy upgrading of your old units, Galileo flush-cut trays and multi-casters are also compatible with gel units from many other manufacturers. All Galileo trays feature multiple comb slots, built-in fluorescent rulers and ultra-strong assembly methods for long, reliable service.
Models
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Model 1010 |
Model 2010 |
Model 1614 |
Model 2020 |
| Description |
Mini twin Vertical |
Wide twin mini vertical |
Midi twin vertical |
Large twin vertical |
| Applications |
Run either one or two gels simultaneously on these versatile separation systems. |
Run either one or two extra wide gels simultaneously on this high capacity separation system. |
Run either one or two large format gels simultaneously on this high capacity separation system.2 |
Run either one or two extra wide long-run gels on this high capacity separation system. |
| Unit dimensions (cm) |
17W × 14H × 12D |
28W × 15H × 13D |
21W × 24H × 14D |
24W × 31H × 17D |
| Gel size (cm) |
10W × 10L |
20W × 10L |
16W × 14L |
20W × 20L |
| Buffer capacity |
400 mL |
850 mL |
700 mL |
1300 mL |
| Acrylamide gel volume |
6-15 mL |
15-30 mL |
14-27 mL |
25-49 mL |
| Max sample capacity/gel |
15 |
36 |
24 |
25 |
| Max sample capacity/unit |
30 |
72 |
48 |
50 |
| Current requirement |
15 – 35 mA per gel |
15 – 45 mA per gel |
15 – 50 mA per gel |
15 – 75 mA per gel |
| run time |
30 – 60 min |
30 – 90 min |
60 – 120 min |
60 – 180 min |
| Standard features |
Platinum electrodes, non-slip feet, optional cooling chamber |
| Safety features |
Safety cover, sheathed power leads |
| Included glass plates |
4 plain 10 × 10 × 0.24 cm
2 notched 10 × 10 × 0.24 cm |
4 plain 20 × 10 × 0.32cm
4 notched 20 × 10 × 0.32 cm |
4 plain 16 × 14 × 0.32cm
4 notched 16 × 14 × 0.32 cm |
4 plain 10 × 10 × 0.24 cm
2 notched 10 × 10 × 0.24 cm |
| included combs |
2 × 10 well @ 0.8 mm |
2 × 15 well @ 0.8 mm
2 × 20 well @ 0.8 mm |
2 × 10 well @ 1.5 mm
2 × 15 well @1.5 mm |
2 × 15 well @ 1.5 mm
2 × 20 well @1.5 mm |
| Included spacer |
4 @ 0.8 mm |
8 @ 0.8 mm |
8 @ 1.5 mm |
8 @ 1.5 mm |
Frequently Asked Questions
Polyacrylamide (Vertical) gels
When using vertical gel electrophoresis devices, spacers are sandwiched between one notched and one plain glass to form the gel “cassette”. The notched glass plate provides a contact are for the buffer from the upper buffer chamber to flow over the gel to complete the electrical circuit required for electrophoresis. Note that the “ears” on notched plates are fragile, and should be handled with care. Extra notched and plain glass plates are available for all Galileo vertical units.
If there are voids at the base of a vertical gel, bubbles can be trapped when the gel cassette is placed in the running buffer. These bubbles will cause an uneven electrical field and poor gel performance. Galileo vertical gel systems feature a cast-in-place system that that forms a smooth, flush seal at the bottom of the gel cassette. This allows for full filling and polymerization (no oxygen exposure) along the bottom edge of the gel, preventing any voids which would trap air and cause bottom bubbles.
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