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Centrifuge 5804 / 5804 R
Centrifuge 5804 / 5804 R is a high speed centrifuge for medium capacity needs. It allows for molecular applications in tubes up to 250 mL and offers additional swing–bucket and fixed–angle rotors as well as deepwell plate capacity for increased versatility.
The bench-top centrifuges from Eppendorf are long established and enjoy a popularity that grows with every passing year. Thus, it was natural for Eppendorf to take the new technological possibilities into account and subject their popular Centrifuges 5804 (refrigerated: 5804 R) as well as the 5810 (refrigerated: 5810 R) to a live-cell treatment. The result is a device with the same chassis, but with a considerably improved control panel and an inner life incorporating state of the art technology.
The comprehensive, modular accessory program covers separation in PCR tube strips and 1.5/2 mL test tubes as well as high-volume centrifugation of Falcon®tubes, flasks and filter plate systems and therefore practically all refrigerated and non-refrigerated applications up to 500 mL volume per tube (5810/R only).
Eppendorf Multipurpose Centrifuges feature powerful, maintenance-free motors, and they accommodate a variety of rotors for unparalleled application versatility. They are three centrifuges in one:
A high capacity general purpose centrifuge for cell harvesting
A high-speed centrifuge for separating cell lysates
A micro centrifuge for DNA precipitations.
Multipurpose Centrifuge Models 5804 and 5804R Features
Interchangeable swing-bucket and high-speed fixed-angle rotors spin microcentrifuge tubes, popular glass and plastic tubes, microplates, and blood collection tubes
Variable speed, maintenance-free motor spins up to 14,000 rpm, 20,800 ×g
Low profile 11-inch access height for easy sample loading
Automatic rotor recognition senses rotor type to set maximum allowable speed – ensures safety
Simple, easy-to-use programming:
Time, temperature, speed/g-force
34 user-defined programs with write protection for quick parameter entry ensures reproducibility
Adjustable-speed Short Spin button
Ten acceleration and braking rates – ideal for protecting sensitive samples
Cooling features of the 5804R Refrigerated Centrifuge
Fast Cool function pre-cools chamber in as little as 15 minutes
Temperature range from -9 °C to 40 °C
Standby refrigeration keeps rotor chamber at set temperature while lid is closed
User-friendly operation
Speed, rcf, and radius correction values can be entered by the user
Values can be changed during centrifugation
Temperature range from -9 to +40 °C*
Stand-by refrigeration*
Speed can be set from 200 rpm to maximum speed in increments of 10 rpm
Selectable program memory for up to 34 individual user programs
Short-spin key with selectable rotational speed
Fast-temp function* for rapid cooling of the centrifuge chamber
"at set rpm" for even more reproducable results
10 acceleration and 10 braking ramps for sensitive sample material
*Refrigerated Centrifuges 5804 R and 5810 R only.
Technical Information
5804
5804 R
Max. RCF
20,913 × g
20,913 × g
Max. RCF with fixed-angle rotor
20,913 × g
20,913 × g
Max. RCF with swing-bucket rotor
4,500 × g
4,500 × g
Max. RCF with plate rotor
2,250 × g
2,250 × g
Speed
200 – 14,000 rpm (10 rpm steps)
200 – 14,000 rpm (10 rpm steps)
Max. capacity
4 × 250 mL/
2 × 5 MTP
4 × 250 mL/
2 × 5 MTP
Rotors available
12
12
Acceleration/braking ramps
10/10
10/10
Number of programs
35 user-defined programs
35 user-defined programs
Timer
1 min to 99 min, with continuous run function, short-spin
1 min to 99 min, with continuous run function, short-spin
No, the Centrifuges 5804/ 5804 R and 5810/ 5810 R do not come with a transport protection device. They are packaged in polystyrene for protection. Because the rotor is not in the centrifuge during transport, there are no movable parts to make a transport protection device necessary.
Yes, the speed, the time and the temperature can be changed even when the centrifuge is running. To do this, please press the SPEED, TIME or TEMP key and change the values by pressing the arrow keys.
The minimum rotational speed is 200 rpm, irrespective of the inserted rotor. The minimum rotational speed can be increased in increments of 10 rpm up to 5,000 rpm and then in increments of 100 rpm.
This display appears every 200 centrifugation runs. For cleaning the rotor and the rotor chamber, the rotor must first be removed from the centrifuge. To do this, loosen the rotor nut by turning it counter-clockwise with the supplied rotor key. Please clean the rotor and rotor chamber only with mild cleaning agents (e.g. 70% alcohol solutions or commercially available alcoholic disinfectants).
All data is saved that can also be entered manually, such as rotational speed or RCF, maximum radius, run time, acceleration and braking ramps, "at set rpm", temperature.
Yes, it is possible, provided that the rotor is loaded symmetrically. This means that the 50 mL tubes and the 15 mL tubes are positioned opposite one another and are positioned symmetrically. Please use the appropriate adapters for the tubes.
50 mL DIN glass tubes (diameter 34 mm, length 100 mm) can be centrifuged in the adapter for 50 - 75 mL tubes (order number 5804 760.000) of the swing-bucket rotor A-4-44 or adapter 5810 760.003 of swing-bucket rotor A-4-62 . Depending on the length of the tube, the uppermost module of the adapter can be removed for centrifugation of 50 mL DIN glass tubes.
With a rubber adapter (outer diameter 18 mm) available from the tube manufacturer, the 15 mL Corex tubes fit into the adapter 5804 772.008 of the rotor F-34-6-38. With a rubber adapter (outer diameter 29 mm) available from the tube manufacturer, the 30 mL Corex tubes fit into the adapter 5804 774.000 of the rotor F-34-6-38. This rotor can be inserted into the Centrifuge 5804/5804 R or 5810/5810 R.
Up to 12 slides can be inserted into the CombiSlide adapter and then centrifuged in the swing-bucket rotors of Centrifuge 5804/ 5804 R or 5810/ 5810 R.
This label states that the rotor can be loaded with a maximum of 6 ×125 gram. "g" is the official abbreviation for "gram". Where "g" is used as an abbreviation of "g-force", the number and "x g" are used. Example: 10,800 ×g.
Yes, you can use the new rotors from software version 8.4 onwards. From versionb 7.0 on a software update to version 8.4 can easily be performed by trained service technician. In case of older software versions, the complete PCB control/frequency converter must be replaced. You can see the current software version of your centrifuge briefly on the display after switching it on.
A new rotor automatically will be recognized as soon as it starts moving. Moving and recognition of the rotor can be performed manually by making it spin counterclockwise (roughly 200 rpm necessary), or by turning the Centrifuge on. As soon as the rotor starts spinning it will be recognized. When indicated, the Centrifuge shuts down under specification of max. speed in case the indicated max. speed of the previous rotor was higher. In this case the Centrifuge must be restarted.
