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All equipment servicing to manufacturer’s specs by Eppendorf factory certified technicians. Calibration, repair, service, spare parts, Compliance Covering - IQ/OQ/PQ, calibration and validations. On-site repairs are available for customers in Sydney, Melbourne and Brisbane. For other areas/States, we offer a workshop service or spare parts.

Spectrophotometer (spectro) Eppendorf's BioPhotometer plus ~ overview

These are POCDS Time Critical Products - we are seriously committed to having these products in stock 24/7/365 for immediate shipping. If we are unable to immediately dispatch any of these items to you upon receiving your order we will airfreight the item from Germany at our expense to minimise the delay in getting the item to you.

With the stand-alone spectrophotometer, the BioPhotometer plus, Eppendorf offers a compact fully self-contained UV/Vis spectrophotometer for use in molecular biology, biochemistry and cell biology.

The photometer provides instant, out-of-the-box access to 32 routine methods, of which 9 methods are freely programmable, eg:

• Measurement of DNA, RNA and protein concentration (UV and colorimetric) - also known as dna quantitation, rna quantitation and protein quantitation
• Incorporation rate of fluorescent molecules (550 nm/650 nm), eg for microarray experiments
• Enzymatic assays (e.g., peroxidase, alkaline phosphatase, β-galactosidase)
• Optical density of cells (OD 600)

Both measurement (quantitation) and calculation of results are performed at the press of a button, and the results of the spectrophotometric analysis, as well as all accompanying quantitaion data, are available at a glance. This guarantees safe, error-free operation and reliable results.

In addition to its low weight, the spectrophotometer is small, yet extremely sturdy due to a robust metal housing, which allows for easy transport and cleaning.

Eppendorf's stand-alone spectrophotometer has practical advantages over such popular spectrophotometers as the nanodrop and nanophotometer. Whereas the nanodrop and nanophotometer require connection to a computer and specific software to carry out the spectrophotometric analysis, Eppendorf's Biophotometer plus carries out the quantitation analysis by itself

The BioPhotometer plus is designed for use with cuvettes such as the Eppendorf UVette^®, but is also optimized for measurement of small volumes through the use of microliter cells such as Hellma^® TrayCell.

The new BioPhotometer data transfer software can now be used to download/transfer all measurement, quantitation and calibration data to a PC. Besides enabling the printing of results it also allows results to be exported to data processing software for further evaluation.

The program also offers the option of data archiving, which can be clearly configured with an optional user administration.

Spectrophotometer (spectro) Eppendorf's BioPhotometer plus ~ features

Applications

• Quantification / quantitation of nucleic acids (dsDNA, ssDNA, RNA, Oligo)
• Quantification / quantitation of proteins (280 nm, Bradford, Lowry, BCA)
• Cell density measurement (OD600)
• NEW - Incorporation rate of fluorescent dyes (dye 550/650, e.g., Cy-3, Cy-5)
• NEW - Enzymatic assays: endpoint detection (e.g., of enzyme activities or cell biological assays)
• NEW - Direct absorbance measurement of single wavelengths without any calculation (230 nm, 260 nm, 280 nm, 340 nm, 405 nm, 490 nm, 550 nm, 595 nm, 650 nm)

Features

• Fully self-contained - does NOT require PC to function
• 9 wavelengths suitable for the common methods in molecular biological, biochemical and cell biological labs
• Preprogrammed methods for quantification of nucleic acids and proteins as well as incorporation rate of fluorescent dyes
• Automatic calculation of dilution factors
• Assays at 340 nm, 405 nm, and 490 nm are user defined for highest flexibility
• Measurement of single wavelengths without any calculation
• Storage of the last 100 results and all corresponding data
• Simple user guidance for error-free operation
• Different colored function keys for easy operation
• Compact design and robust housing
• Stand-alone unit without need for PC connection
• No pre-warming saves time
• Xenon flash lamp with long lifetime and high light intensity
• No moving parts – less service needs

Spectrophotometer (spectro) Eppendorf's BioPhotometer plus ~ technical

Optical system:	Absorption singlebeam photometer with reference beam
Light source:	Xenon flash lamp
Light beam height:	8.5 mm
Measuring wavelengths:	230, 260, 280, 340, 405, 490, 550, 595, 650 nm
Spectral bandwidth:	5 nm at 230–340 nm, 7 nm at 405–650 nm
Wavelength systematic error:	±1 nm at 230–280 nm, ±2 nm at 340–650 nm
Photometric measuring range:	0 to 3 A (2 A at 340 nm); Dye Methods: 2 A at 550 nm/650 nm
Photometric random error:	±0.002 A at 0 A; ±0.005 A at 1 A
Photometric systematic error:	±1 % at 1 A
Method dependent calculation:	Absorbance Concentration via factor Concentration via calibration with 1 to 10 standards One point calibration (1 standard) Linear regression (2 to 10 standards) Nonlinear regression (3rd degree polynom; 4 or 5 to 10 standards), 1 x, 2 x or 3 x determination Ratio 260/280, ratio 260/230, molar concentration, total yield For dye methods: FOI (frequency of incorporation)
Calibration memory:	For all calibration procedures
Results memory:	For 100 results with absorbance and ratio values, sample number, sample dilution, date and time
Interface:	RS232C, serial, PC connection optional
Power requirement:	Approx. 20 W in operation, approx. 10 W in Standby mode
Power supply:	100–240 V, ±10 %; 50–60 Hz, ±5 %
Dimensions (W x D x H):	20 x 32 x 10 cm
Weight:	3 kg
Accessories:	Thermal printer DPU 414, 40 characters/line; Secondary UVVIS filter set for verifying photometric and wavelength accuracy (NIST traceable)
Cuvettes:	UVette, Hellma TrayCell*, and common rectangular cuvettes of suitable glass or plastic materials

Spectrophotometer (spectro) Eppendorf's BioPhotometer plus ~ ordering

Please note:

We have endeavoured to provide Australian catalogue numbers; however it would be prudent to verify any catalogue numbers taken straight from our site with POCD Scientific before using them to place an order. We are seriously committed to having these product in stock 24/7/365 for immediate shipping. However we do need to acknowledge that spikes of demand can temporarily deplete our normally robust stock levels. If such a situation affects your order we will airfreight the item from Eppendorf at our expense to minimise the delay in getting the item to you.

Description	Order no.
BioPhotometer plus, 230 V/50–60 Hz	6132 000.008
Secondary UV-VIS filter test set for verifying photometric precision and wavelength accuracy (NIST traceable)	6131 928.007
Thermal printer DPU 414, incl. power supply 230 V and printer cable	6131 011.006
Thermal paper (5 rolls)	0013 021.566
UVette®, individually packaged single cuvettes, certified RNase, DNA and proteinfree, 80 pcs.	0030 106.300
UVette routine pack, Eppendorf Quality purity level, reclosable box, 200 pcs.	0030 106.318
BioPhotometer Data Transfer Software	6132 854.007

Spectrophotometer (spectro) Eppendorf's BioPhotometer plus ~ consumables

The Eppendorf UVette^® fulfils the highest specifications of modern spectrophotometric analysis in the UV range and can be optimally used for sensitive samples and small volumes.

Spectrophotometer (spectro) Eppendorf's BioPhotometer plus ~ the Uvette adapter

Description of the Adapter

The UVette is ideal for use with the Eppendorf BioPhotometer plus. In other common photometers and/or spectrophotometers, the UVette is inserted with the aid of an adapter, which performs two functions:

• It aligns the UVette to the height of the photometer’s light beam
• Diaphragm function for an optimal distribution of light

Compatibility list:
Which adapter for which device?

Manufacturer	Device	UVette adapter/size	Comments
Varian	Cary 50	15 mm	Proven by customers.
	Cary 100	8.5 mm	-
GE Healthcare	GeneQuant® I/II	GeneQuant I/II	-
	GeneQuant devices other than GeneQuant I/II	15 mm	-
	Ultrospec series	15 mm	-
	GeneQuant™ 1300	15 mm	-
Agilent/ Hewlett Packard	8452A UV-Vis spectroscopy system	15 mm	Proven by customers
Agilent Technologies	8453 UV-Visible Spectrophotometer	15 mm	Proven by customers.
Analytik Jena	SPECORD series	8.5 mm or 15 mm	When using the adjustable cuvette holder for 8.5 mm or 15 mm light path height.
	SPEKOL series	8.5 mm	When using the adjustable cuvette holder for 8.5 mm or 15 mm light path height.
Biochrom®	Libra S21/S22	15 mm	-
	Libra S32/S32PC	15 mm	-
Eppendorf	BioPhotometer / BioPhotometer plus	-	No adapter is required.
Hitachi®	UV-series	UBA 410–1500	Only available in Europe. For further information: support@eppendorf.com
	Hitachi U1800	15mm	Proven by customers.
	Hitachi U2900	10mm	Proven by customers.
	GeneSpec I/II	UBA 410–1500	Only available in Europe. For further information: support@eppendorf.com
Implen	NanoPhotometer™	15mm	-
Jenway	Genova	Adapter from Jenway	Please contact your local Techne or Jenway representative.
PerkinElmer®	Lambda series, except EZ series	15 mm	-
	Lambda EZ 201 and EZ 210	8.5 mm	When using the micro cell holder.
Secomam	UVIKON series	15 mm	When using the single-cell holder. For models 943 and XL, the 15 mm adapter can also be used for multi-cell holder 6 x 6.1.
Shimadzu®	UV-series	15 mm	When using the standard cell holder.
	UV Multispec-1500	15 mm	-
	BioSpec®	15 mm	-
Techne®	Genova	Adapter from Jenway	Please contact your local Techne or Jenway representative.
	Specgene	Adapter from Jenway	Please contact your local Techne or Jenway representative.
Thermo Fisher Scientific	BioMate series	15 mm	When using the single-cell holder.
	Helios series	15 mm	When using the single-cell holder.
	Genesys 10S	-	Proven by customers.
	NanoDrop 2000c	-	See recommendations NanoDrop 2000/ 2000c User Manual (V 1.0).

This adapter list provides an overview of the correct adapter for use with Eppendorf’s UVette® in devices from other manufacturers. No adapter is required for use in Eppendorf’s photometers.

The adapters’ compatibility with the devices from other manufacturers has been jointly tested by Eppendorf, the device manufacturers and our customers.

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