Eppendorf Tubes are the benchmark for simple and safe sample preparation from 0.5 to 2.0 mL. For larger volumes, 15 mL and 50 mL conical tubes are available.
With medium sample volumes you may face an issue: they need to be processed with large conical screw cap tubes - impractical, inconvenient and often prone to contamination.
Eppendorf now offers the "missing link": the Eppendorf Tube 5.0 mL. This product fills the gap between existing tube versions and enables the simple and safe processing of sample volumes up to 5.0 mL!
Easier processing of samples up to 5.0 mL
Higher yields in DNA isolation, especially with samples with limited quantity of source material
Convenient and safe cell culture applications
Easier sample access lowers the risk of contamination
Preparation of mastermixes and buffers
Less storage space needed
Safe cell and tissue lysis
Eppendorf 5.0ml tubes ~ features
Simple, practical and ergonomic single-hand operation. Large labeling area.
Hinged lid for minimized sample evaporation during storage and incubation in a wide range of temperatures from -86 Â°C to 80 Â°C
Exceptionally high-quality, transparent polypropylene, free of plasticizers, biocides or mold release agents, for reliable test results
Available in lot-tested and certified Eppendorf Quality, PCR clean, Sterile and Eppendorf BiopurÂ® purity levels
Maximum safety and stability for centrifugation up to 25,000 Ă— g and fast and efficient protocols.
Compatible with accessories for conical 15 mL tubes - many existing adapters and racks can be used.
Available in Eppendorf LoBindÂ® material for maximum recovery of valuable samples
Eppendorf 5.0ml tubes ~ ordering
We have endeavoured to provide Australian catalogue numbers; however it would be prudent to verify any catalogue numbers taken straight from our site with POCD Scientific before using them to place an order.
Eppendorf Protein LoBind Tubes 5.0 mL, PCR clean, 100 tubes (2 bags of 50 ea.)
Eppendorf DNA LoBind Tubes 5.0 mL, PCR clean, 200 tubes (4 bags of 50 ea.)
Starter Pack of Eppendorf TubesÂ® 5.0 mL, PCR clean, 400 tubes (2 packages with 2 bags of 100 ea.), 2 racks (with 16 spaces), white, universal adapter for rotors with bore for 15 mL conical tubes (8 pcs.)
Rack 5.0 mL (16 spaces), 2 pcs., white, also suitable for 15 mL conical screw cap tubes
Storage box 5.0 mL (25 spaces), 2 pcs., 5 Ă— 5 grids, blue and red, made of polypropylene for freezing up to â€“86 Â°C, with lid and alphanumeric code, height: 67 mm
Tube Clip 5.0 mL, 10 pcs. , secures lid for boiling
Centrifuge 5427 R: rotor
Rotor FA-45-12-17, for 12 Ă— Eppendorf TubesÂ® 5.0 mL, up to 20,600 Ă— g, aerosol-tight
Centrifuge 5430/5430 R: rotors and adapters
Rotor FA-45-16-17, for 16 Ă— Eppendorf TubesÂ® 5.0 mL, up to 21,191 Ă— g, aerosol-tight
Adapter for Rotor F-35-6-30 (large bore), 2 pcs., for 2 Ă— Eppendorf TubesÂ® 5.0 mL, up to 7,129 Ă— g
Adapter for Rotor F-35-6-30 (small bore), 2 pcs., for 2 Ă— Eppendorf TubesÂ® 5.0 mL, up to 6,237 Ă— g
Centrifuge 5702/ 5702 R/ 5702 RH: adapter
Adapter for Rotor A-4-38, 2 pcs., for 2 Ă— Eppendorf TubesÂ® 5.0 mL, up to 2,943 Ă— g
Centrifuge 5804/ 5804 R and 5810/ 5810 R: rotors and adapters
Rotor FA-45-20-17, for 20 Ă— Eppendorf TubesÂ® 5.0 mL, up to 20,913 Ă— g, aerosol-tight
Adapter for Rotor F-34-6-38 (large bore), 2 pcs., for 2 Ă— Eppendorf TubesÂ® 5.0 mL, up to 16,824 Ă— g
Adapter for Rotor F-45-6-30 (large bore), 2 pcs., for 2 Ă— Eppendorf TubesÂ® 5.0 mL, up to 19,806 Ă— g
Adapter for Rotor S-4-104 (large bore), 2 pcs., for 28 Ă— Eppendorf TubesÂ® 5.0 mL, up to 3,214 Ă— g
Adapter for Rotor S-4-72, 2 pcs., for 16 Ă— Eppendorf TubesÂ® 5.0 mL, up to 3,215 Ă— g
Centrifuges with rotors for 15 mL conical screw cap tubes: universal adapter
Insert, 8 pcs., for 8 Ă— Eppendorf TubesÂ® 5.0 mL
ThermomixerÂ® comfort and ThermoStat plus: thermoblock 5.0 mL, for 8 Ă— Eppendorf TubesÂ® 5.0 mL
Eppendorf ThermoMixerâ„˘ C and ThermoStat C: SmartBlock 5.0 mL, for 8 Ă— Eppendorf TubesÂ® 5.0 mL
epMotionÂ®: Reservoir Rack Module TC 5.0 mL, for 4 Ă— Eppendorf TubesÂ® 5.0 mL
Eppendorf 5.0ml tubes ~ Tube Racks, storage boxes and clips for 5.0 mL
Eppendorf Tube Racks
Eppendorf Tube Racks are ideal for sample preparation and short-term or mid-term sample storage. They can be stored in the freezer and can withstand temperatures up to -86 Â°C. The individual tubes are carefully arranged and positioned higher in the rack so they are easy to access and support ergonomic working methods. Tube Racks are available for 0.5 mL, 1.5 mL, 2.0 mL and 5.0 mL vessels. They can be stacked and are labeled for easy identification. The Tube Racks are UV-resistant and fully autoclavable.
Storage Box 5.0 mL
Storage box 5.0 mL (25 spaces), 5 Ă— 5 grid, blue and red, made of polypropylene for freezing up to â€“86 Â°C, with lid and alphanumeric code, height: 67 mm
Tube Clip for Eppendorf TubesÂ® 5.0 mL
A small additional safety clip that prevents tube opening in boiling applications (85Â°C -100Â°C)
Eppendorf 5.0ml tubes ~ downloads
Eppendorf 5.0mL Tubes - The Missing Link
This is a PDF file, to obtain a free Adobe PDF reader:
What types of applications can be carried out with Eppendorf TubesÂ® 5.0 mL?
Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL.
The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades â€śSterileâ€ť (= sterile and pyrogen-free) or Biopur. They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.
Is it necessary to buy new equipment to be able to use the new tube?
The bottom shape of the Eppendorf TubesÂ® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices.
Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotionÂ® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265.
What are the advantages of using Eppendorf TubesÂ® 5.0 mL compared with conical 15 mL screw-cap tubes?
The compact, space-saving design greatly facilitates the processing of samples with volumes in the range of 2-5 mL. Eliminating the risk of dipping the tip cone into the tube avoids contamination. The snap-cap lid allows one-hand operation and the tubes can withstand forces of up to 25,000 x g for time-saving and effective centrifugation. A further advantage is that no components are released from the material of the Eppendorf Tubes which could affect photometric detection methods (Application Note 264). A lot of information on using the tubes can be found in Application Note 265.
Is it possible to store samples in 5.0 mL Eppendorf TubesÂ® in the freezer?
Yes, the tubes can be stored down to -86 Â°C.
How resistant are the Eppendorf TubesÂ® 5.0 mL to chemicals?
The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.
Are the Eppendorf TubesÂ® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?
The Eppendorf TubesÂ® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and BiopurÂ® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.
Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?
It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.
What is the Tube Clip used for?
The Eppendorf TubesÂ® 5.0 mL offer secure closure at incubation temperatures up to 80 Â°C. For higher temperatures, the Tube Clip should be used to secure the lid.
Up to which g-force can the Eppendorf TubesÂ® 5.0 mL be centrifuged?
All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45Â° fixed-angle rotor at 40 Â°C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.
Eppendorf 5.0ml tubes ~ video footage
Some video showing you the Eppendorf 5.0ml tubes in actioncourtesy Eppendorf