The Eppendorf BioPhotometerĀ® D30 is the 3rd generation of Eppendorf BioPhotometers, which have become an established standard in the field of life sciences.
The measurement data will be recorded for fixed wavelengths, making the BioPhotometerĀ® D30 the ideal device for routine applications.Data are clearly processed making the evaluation of results fast and simple while minimizing the risk of mistakes. Additional assistance for the user, including comparative spectra that are stored in the device, offer an additional level of security. Furthermore, all relevant ratios are automatically determined for the corresponding applications.
The BioPhotometerĀ® D30 offers the option of recording purity scans for specific applications. In a defined measuring range, additional measurement data is collected and shown extrapolated, allowing contamination in the sample to be visually identified quickly.
Help box with explanation of each individual step in 5 languages
Compatible with microvolume measuring cells and standard cuvettes
Absorption measurements for one or more fixed wavelengths
Display of purity scans for specific applications
Applications with evaluations via factor, standard or standard series
Eppendorf BioPhotometer D30 ~ technical
Dimensions (W × D × H)
295 × 400 × 150 mm
Weight w/o accessories
100-240 V/50-60 Hz
USB master: for USB stick
USB slave: for connecting to a PC
Interface for Eppendorf thermal printer: serial RS232
5.7” VGA TFT display
For measurements in the UV range: quartz glass or UV-transparent plastic (Eppendorf UVette®, 220 nm to 1,600 nm)
For measurements in the visible range: glass or plastic
For microvolume measurements: microvolume measuring cells such as the Eppendorf μCuvette™ G1.0
12.5 mm × 12.5 mm, untempered
Light path height
Xenon flash lamp
Absorption measuring range
0 A – 3,0 A (260 nm)
Operator guidance language
Spanish, Italian, French, English, German
± 0.5 nm
≤ 4 nm
± 1 nm
230, 260, 280, 320, 340, 405, 490, 562, 595, 600
Eppendorf BioPhotometer D30 ~ ordering
We have endeavoured to provide Australian catalogue numbers; however it would be prudent to verify any catalogue numbers taken straight from our site with POCD Scientific before using them to place an order.
BioPhotometer D30 reference filter set
filter set for verifying photometric accuracy and wavelength systematic error (according to NIST)
Eppendorf BioPhotometer D30 ~ accessories
Eppendorf Ī¼Cuvette™ G1.0
With an optical path length of only 1 mm, the Ī¼Cuvette G1.0 features a light path
that is ten times shorter than the light path on standard cuvettes. This allows high
nucleic acid and protein concentrations to be measured using the Ī¼Cuvette G1.0.
Only 1.5 Ī¼L of a sample is required for the measurement. The Ī¼Cuvette G1.0
offers a flexible expansion of the photometric application range of Eppendorf
Eppendorf Ī¼Cuvette™ G1.0 benefits
Microvolume measuring cell for measuring 1.5 ā10 Ī¼L sample volumes
Concentration determination of nucleic acids and proteins
Measurement of high sample concentrations without prior dilution
Exclusively available for Eppendorf BioPhotometer and Eppendorf BioSpectrometer
UV/Vis BioSpectrometer reference filter set
The UV/Vis BioSpectrometer reference filter set is used to determine the photometric accuracy and wavelength systematic error in accordance with NIST. It is for use with the BioSpectrometer basic and BioSpectrometer kinetic.
Filter traceable to NIST
Certificate with two year period of validity
Filter recertification via Eppendorf (Contact: Eppendorf Service)
Inspection protocol is preprogrammed in device
Checking wavelength systematic error and precision at 260 nm, 280 nm and 800 nm
Checking photometric accuracy and precision in the 260 nm to 800 nm range
The variation coefficient is listed along with the average value of 15 measurements.
Includes 7 filters (1x empty value filter, 3x filters for checking the wavelength systematic error and precision, 3x filters for photometric testing)
Eppendorf BioPhotometer D30 ~ consumables
The Eppendorf UVette® fulfils the highest specifications of modern spectrophotometric analysis in the UV range and can be optimally used for sensitive samples and small volumes.
Is it possible to use the Eppendorf ĀµCuvette also in the BioPhotometer D30?
Yes, this is possible without any restrictions
What is the minimum volume for nucleic acid solution that I can use in the Eppendorf ĀµCuvette that I can use in the BioPhotometer D30?
The smallest volume should be at least 1.5 ĀµL.
What is the minimum volume for protein solution that I can use in the Eppendorf ĀµCuvette that I can use in the BioPhotometer D30?
This depends also to some extend to on the proteins. We recommend a volume of at least 3 ĀµL.
Is it possible to carry out a wavelength scan with the BioPhotometer D30?
No, this is unfortunately not possible. The BioPhotometer D30 is a fix wavelength photometer for specific methods.
What wavelength could be applied in the BioPhotometer D30?
The wavelengths 230, 260, 280, 320, 340, 405, 490, 562, 595 und 600 nm could be applied.
What methods are preprogrammed in the BioPhotometer D30?
Preprogrammed are all nucleic acid and protein detection methods and the OD 600 method for the determination of the optical density of bacterial or yeast cultures. Protein can be detected at 280 nm and via colorimetric assays like the BCA, the Bradford or the Lowry method.
Is it possible to use the BioPhotometer Data Transfer Software for the BioPhotometer D30?
No, this is unfortunately not possible. But this is also not necessary since the results are exported directly as Excel-files. An additional program for data management is therefore not necessary.
What can I do with the so called "impurity scan"?
With the "impurity scan" the absorbance of a certain wavelength range is shown for nucleic acid and protein measurements. With this function impurities in the sample can be easily detected via deviations from the known curve.
For what methods the "impurity scan" could be applied?
This function is available for nucleic acid and protein detections.
What wavelength range is used for the "impurity scan"?
For protein measurements it is the wavelength range from 245-350 nm and for nucleic acids the range from 225-350nm.
Is it possible to change the range for the "impurity scan" and can I display the single absorbance values of the "impurity scan"?
No, unfortunately both options are not possible. This can be done only in the BioSpectrometer
Can I use the HellmaĀ® TrayCell with the BioPhotometer D30?
Yes, this is of course possible! Please check, that the cuvette with the required light beam hight of 8.5 mm is applied. Furthermore it is necessary for the precise calculation of the sample concentration that the correct light path length is set in the parameter section (stated on the lid)
What kind of cuvette path lengths could be applied for the BioPhotometer D30?
Like for the BioSpectrometer the cuvette path lengths of 10, 5, 2, 1, 0.2, 0.1 mm could be programmed.
Is there any possibility to check the BioPhotometer D30?
Yes, this is possible. We offer a calibration filter set especially for the BioPhotometer D30.
In what file-format are the files from the BioPhotometer D30 exported?
The data will be exported as Excel-files
Is it possible to select my data for the export?
Yes, this is possible! In the method you can select in the section "print&export" specific data packages and specific results for the export.
What kind of SI-units can be applied with the BioPhotometer D30?
This depends on the method. The free programmable methods offer 18 different kind of SI units. But it is possible at any time to edit new SI-Units?
In the BioPhotometer D30 certain factors are preprogrammed for the evaluation of specific proteins. Is it possible to apply factors also for other proteins?
Yes, this is possible. Select in the "parameter" section of the method "protein". With "edit" a calculation tools for protein factor starts. Here, you may set the factor for the new protein directly. It is also possible to calculate the factor via the absorbance coefficient and the molecular mass of the protein or via its value "A0.1%"
What is the meaning of the value "A0,1%"?
The meaning of A0,1% is the absorbance of the Protein with a concentration of 1mg/ml.
How many methods can be stored in the BioPhotometer D30?
About 100 methods can be stored in the BioPhotometer D30 or the BioSpectrometer.
How many results can be stored in the BioPhotometer D30?
About 1000 results can be stored in the BioPhotometer D30 or the BioSpectrometer.